| 作者 |
Xiang QIN, Xiaoni PAN,Yuan PAN, Yanfeng MO, Shuwen LI, Tingting XIE, Shiyou ZHOU, Jiancheng LIANG, Lixiang LU, Suoyi HUANG |
| 摘要 |
[Objectives] To explore the efficacy of different concentrations of Centipeda Herba volatile oil in the treatment of allergic rhinitis (AR) by nasal sniffing and the optimal drug concentration. [Methods] Forty-eight mice were randomly divided into control group, blank group, alcohol treatment group, low concentration group, medium concentration group and high concentration group, with 8 mice in each group. Except for the blank group, each mouse in the other groups was intraperitoneally injected with 1 mL of a mixture of OVA 50 μg + [Al(OH)3] 5 mg + 1 mL of normal saline for 14 d. The allergic rhinitis mouse model was successfully established by intranasal instillation of 5% OVA solution on both sides (20 μL per side, once a day) from the 15th day after stimulation for 7 d. The blank group was treated with the same amount of saline as above. The volatile oil of Centipeda Herba was obtained by steam distillation and petroleum ether extraction, and then was made into 1.25%, 2.5% and 5% volatile oil of Centipeda Herba with 75% alcohol. The control group was stimulated once every other day with reagent 2 after 7 d of stimulation (maintenance) until the end of treatment. The blank group was treated with 5 ml saline by nasal sniffing for 30 d, twice in the morning and evening, 30 min each time. The alcohol treatment group was treated with 5 mL of 75% alcohol, and the low concentration group, the medium concentration group and the high concentration group were treated with 5 mL of 1.25%, 2.5% and 5% Centipeda Herba volatile oil, respectively. The treatment time was the same as that of the blank group, and the treatment process was carried out in their respective closed contamination boxes. Before and after the treatment, the frequency of sneezing, the frequency of scratching nose, the amount of nasal discharge, activity and other general characteristics of the mice were observed, and the allergic behavior score was carried out. Besides, the IgE content in the serum of the mice was determined, and the eosinophils in the nasal discharge were counted. [Results] The scores of mice before and after treatment showed that there was no significant difference in the alcohol treatment group before and after treatment (P > 0.05), and there was significant difference in the low, medium and high concentration groups before and after treatment (P < 0.05), except that there was no significant difference between the control group and the alcohol treatment group (P > 0.05), and there was significant difference among the other groups (P < 0.05). The levels of IgE and the number of eosinophils in peripheral serum of mice in the control group, alcohol treatment group, low concentration group, medium concentration group and high concentration group were higher than those in the blank group (P < 0.05), and there was no significant difference between the two groups (P > 0.05). [Conclusions] Volatile oil of Centipeda Herba can be used to treat allergic rhinitis by nasal sniffing, and 5% volatile oil of Centipeda Herba has the best effect. During the treatment, sneezing and runny nose in mice were reduced. The results showed that nasal sniffing was less irritating to the nasal cavity and not easy to produce discomfort, the utilization rate of drugs was higher than that of traditional therapy, and the volatile oil could be preserved longer than that of traditional Chinese medicine. |
