| 摘要 |
[Objectives] To investigate the anti-inflammatory effect of Embelia parviflora Wall. polysaccharide on rheumatoid arthritis (RA) in rats. [Methods] RA rat model was induced by type II collagen. After successful modeling, the rats were divided into model group, positive group, low, medium and high dose of E. parviflora Wall. polysaccharide groups, and normal control group. Body mass, toe volume and arthritis index were measured, and thymus index and spleen index were calculated. The levels of interleukin-1β, interleukin-6 and tumor necrosis factor-α in serum and synovial tissue of ankle joint were detected by ELISA. [Results] Compared with the normal control group, the pathological changes such as synovial hyperplasia and unclear layer were observed in the model group, the body mass was decreased (P<0.05), the toe volume, arthritis index, thymus and spleen index were increased (P<0.05), and the levels of IL-1β, IL-6 and TNF-α in serum and ankle synovial tissue were increased (P<0.05). Compared with the model group, the histopathological changes in synovium of ankle joint in the positive group and the medium and high dose groups of E. parviflora Wall. polysaccharide were significantly improved, and the body mass was increased (P<0.05). The toe volume, arthritis index, thymus index and spleen index were decreased (P<0.05). The levels of IL-Iβ, IL-6 and TNF-α in serum and synovial tissue of ankle joint were increased (P<0.05), while there was no significant difference between the low dose group of E. parviflora Wall. polysaccharide and the model group (P>0.05). [Conclusions] E. parviflora Wall. polysaccharide can reduce the body’s inflammatory response and improve RA, which may be related to the inhibition of the activation of inflammatory cytokines IL-1β, IL-6 and TNF-α. |
