| 摘要 |
[Objectives] To analyze the function of cobQ gene from Vibrio alginolyticus strain HY9901, and to provide a reference for exploring the possible mechanism of cobQ gene from V. alginolyticus. [Methods] A pair of primers were designed based on the sequence of the V. alginolyticus cobQ gene and used to amplify the full-length gene by PCR. [Results] The PCR amplification results indicated that the cobQ gene has a full length of 780 bp, encoding 259 amino acid residues. The deduced amino acid sequence predicts a molecular weight of approximately 28.83 kD and an isoelectric point of 9.21. Sequence analysis revealed no N-terminal signal peptide cleavage site, suggesting the absence of both a signal peptide and transmembrane regions in this protein. The amino acid sequence contains 2 N-terminal myristoylation sites, 1 N-glycosylation site, 1 glycosaminoglycan attachment site, 4 microbody C-terminal targeting signal sites, 3 casein kinase II phosphorylation sites, and 4 protein kinase C phosphorylation sites. Subcellular localization prediction showed that the CobQ protein is primarily localized in the cytoplasm (65.2% probability). Homology analysis demonstrated that the amino acid sequence of the cobQ gene from V. alginolyticus shares up to 99% homology with other Vibrio species, clustering within the same subclade as Vibrio parahaemolyticus, indicating close phylogenetic relationships. Secondary structure prediction revealed proportions of α-helices, random coils, and extended strands as 44.40%, 36.68%, and 18.92%, respectively. The tertiary structure model exhibited 87.62% similarity to the template A0A165XBE1.1. [Conclusions] In this study, the V. alginolyticus cobq gene was successfully cloned and its sequence was analyzed by bioinformatics. It is expected to lay a foundation for the subsequent study of the regulatory mechanism of its protein on the virulence of V. alginolyticus. |
