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Reflections on Promoting Rural Development in Mountainous Areas under the "Window of Qingtian" Initiative for New-Era Beautiful Countryside
摘要: Within the context of the "Window of Qingtian" initiative for constructing beautiful countryside in the new era, this study focuses on Malingjiao Village in Zhenbu Town, Qingtian County, exploring innovative approaches and practical models for rural revitalization in mountainous areas. By employing a combination of field research, policy analysis, and case studies, this research deeply analyzes the specific measures implemented in Malingjiao Village, including infrastructure enhancement, integration of primary, secondary, and tertiary industries, revitalization of collective assets, ecological conservation, and cultural heritage preservation. The results indicate that, by pursuing strategies of shared prosperity, agricultural-tourism integration, and integrated development of primary, secondary, and tertiary industries, the village has successfully optimized its industrial structure, expanded the collective economy, increased villager incomes, and improved the ecological environment. This study not only provides a referential demonstration model for beautiful countryside construction throughout Qingtian County but also offers empirical and theoretical support for rural revitalization in mountainous regions across southwestern Zhejiang and even the entire province.
关键词: "Window of Qingtian", Beautiful countryside, Integration of primary, secondary, and tertiary industries, Demonstration model
Cloning and Bioinformatics Analysis of cobQ Gene from Vibrio alginolyticus Strain HY9901
摘要: [Objectives] To analyze the function of cobQ gene from Vibrio alginolyticus strain HY9901, and to provide a reference for exploring the possible mechanism of cobQ gene from V. alginolyticus. [Methods] A pair of primers were designed based on the sequence of the V. alginolyticus cobQ gene and used to amplify the full-length gene by PCR. [Results] The PCR amplification results indicated that the cobQ gene has a full length of 780 bp, encoding 259 amino acid residues. The deduced amino acid sequence predicts a molecular weight of approximately 28.83 kD and an isoelectric point of 9.21. Sequence analysis revealed no N-terminal signal peptide cleavage site, suggesting the absence of both a signal peptide and transmembrane regions in this protein. The amino acid sequence contains 2 N-terminal myristoylation sites, 1 N-glycosylation site, 1 glycosaminoglycan attachment site, 4 microbody C-terminal targeting signal sites, 3 casein kinase II phosphorylation sites, and 4 protein kinase C phosphorylation sites. Subcellular localization prediction showed that the CobQ protein is primarily localized in the cytoplasm (65.2% probability). Homology analysis demonstrated that the amino acid sequence of the cobQ gene from V. alginolyticus shares up to 99% homology with other Vibrio species, clustering within the same subclade as Vibrio parahaemolyticus, indicating close phylogenetic relationships. Secondary structure prediction revealed proportions of α-helices, random coils, and extended strands as 44.40%, 36.68%, and 18.92%, respectively. The tertiary structure model exhibited 87.62% similarity to the template A0A165XBE1.1. [Conclusions] In this study, the V. alginolyticus cobq gene was successfully cloned and its sequence was analyzed by bioinformatics. It is expected to lay a foundation for the subsequent study of the regulatory mechanism of its protein on the virulence of V. alginolyticus.
关键词: Vibrio alginolyticus, Gene cloning, cobQ gene, Bioinformatics analysis
Exploration and Practice of Practical Teaching Pathways for Industry-Education Integration in Environmental Protection in the Context of the Rural Revitalization Strategy
摘要: In the context of the rural revitalization strategy and the "High-Quality Development Project for Hundreds of Counties, Thousands of Towns, and Myriads of Villages", the integration of industry and education in environmental protection majors at universities needs to focus on "demand orientation, technology drive, and collaborative education" as its core. Through curriculum restructuring, joint platform construction, technology empowerment, and policy support, it should form a virtuous cycle of "education chain-industry chain-innovation chain". Under the background of rural revitalization strategy, the present explores practical teaching paths for industry-education integration, including: establishing moral education as the fundamental task for talent cultivation under the rural revitalization strategy; strengthening innovation in curriculum systems and teaching models; implementing a dual-mentor guidance system combining enterprise and academic instructors; adopting comprehensive evaluation systems that integrate theory and practice; improving practical teaching monitoring systems to steadily enhance teaching quality; and effectively strengthening the construction of university-enterprise cooperative bases.
关键词: Rural revitalization, Integration of industry and education, Practice-oriented education