| 刊名 | Agricultural Biotechnology |
| 作者 | Yue ZHANG1,2, Jiawei NIU1,2, Xingsheng LI1, Xiping SUN1, Yangdong GUO1,2*, Xiaowei YUAN1,2,3* |
| 作者单位 | 1. Huasheng Agricultural Group Co., Ltd., Qingzhou 262500, China; 2. College of Horticulture, China Agricultural University, Beijing 100193, China; 3. Weifang Engineering Vocational College, Qingzhou 262500, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.04.004 |
| 年份 | 2025 |
| 刊期 | 4 |
| 页码 | 20-23 |
| 关键词 | Zucchini; Tomato leaf curl New Delhi virus (ToLCNDV); Identification; Sequence analysis |
| 摘要 | [Objectives] This study was conducted to detect and analyze tomato leaf curl New Delhi virus (ToLCNDV). [Methods] Through PCR detection, sequence analysis, and pathogenicity verification, tomato leaf curl New Delhi virus (ToLCNDV) was identified in zucchini exhibiting systemic disease symptoms during a 2024 outbreak in Qingzhou City, Shandong Province, and was designated as ToLCNDV-SD. [Results] Specific primer amplification showed that all eight diseased samples produced bands of 504 bp (DNA-A) and 892 bp (DNA-B). Sequencing analysis revealed that ToLCNDV-SD DNA-A shared 96.10% homology with an Indonesian melon isolate (LC421834.1), while DNA-B showed 88.31% homology with a Malaysian bitter gourd isolate (MW248678.1). Phylogenetic analysis indicated its closest relationship with Southeast Asian cucurbit-infecting isolates. Friction transmission tests confirmed that the virus could spread mechanically, inducing typical symptoms 14 d after inoculation with positive PCR detection. [Conclusions] This study provides important insights for understanding the epidemic mechanisms and control strategies of ToLCNDV in China. |
