| 刊名 | Agricultural Biotechnology |
| 作者 | Hong XUE1#, Chunmei LI1#, Hongyan WU1, Jie LAN1, Jialong LU1, Yiting YANG1,Yujie XU1, Yihe WEI1, Zhiqiang CHEN2*, Xin JIANG2*, Dengfeng ZOU1,3 |
| 作者单位 | 1. College of Pharmacy, Guilin Medical University, Guilin 541199, China; 2. Guangxi Clinical Medical Research Center for Neurological Diseases, First Affiliated Hospital of Guilin Medical University, Guilin 541001,China; 3. Key Laboratory of Early Prevention and Treatment of Regional High Frequency Tumors, Ministry of Education, Guangxi Medical University; Key Laboratory of Early Prevention and Treatment of Regional High Frequency Tumors in Guangxi, Nanning 530021, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.02.001 |
| 年份 | 2026 |
| 刊期 | 2 |
| 页码 | 1-7,16 |
| 关键词 | Human cancer cells; Hypericin; Nanoparticles; Proliferative inhibition; Cytotoxicity |
| 摘要 | To explore the distinct effects of hypericin nanoparticles (HC-NPs) on tumor cell growth, water-soluble HC-NPs were prepared from hypericin and its aqueous solution (HC) and used to treat HepG2 hepatocellular carcinoma cells and HeLa uterine cervical cancer cells. The cell viability of HepG2 and HeLa cells was assessed using the CCK-8 assay, and the IC50 values were determined. The cell migration was evaluated using a scratch wound healing assay to compare the effects of HC-NPs and HC. Experimental findings revealed that both HC and HC-NPs exerted suppressive effects on the vitality of HepG2 and HeLa cells, as indicated by the CCK-8 assay. The IC50 values for HC against tumor cells were identified as 71.00 and 124.35 µg/ml, respectively, while for HC-NPs, these were 27.48 µg/ml for HepG2 cells and 61.00 µg/mL for HeLa cells. The scratch assay demonstrated that HC and HC-NPs effectively hindered cell migration and diminished the rate of wound closure. The live/dead cell co-staining assay further confirmed the suppressive effects of HC and HC-NPs on cell proliferation, with the nanoformulation exhibiting a more pronounced effect. The data suggest that HC-NPs are considerably more effective at inhibiting the proliferation of HepG2 and HeLa cancer cells compared to HC. Subsequently, the binding site of the drug on the cells was identified using laser confocal microscopy. Additionally, flow cytometry and laser confocal microscopy were employed to investigate the generation of reactive oxygen species. In conclusion, the findings of this study highlight the potent inhibitory effects of HC-NPs on the growth of HepG2 and HeLa cancer cells, underscoring their potential as therapeutic agents in cancer treatment. |
