| 刊名 | Agricultural Biotechnology |
| 作者 | Huifang YAO1,Yifan JIA1, Linchen WANG2,Lei WANG1, Zhe YIN1 |
| 作者单位 | 1. Tangshan Food and Drug Comprehensive Inspection and Testing Center, Tangshan 063000, China; 2. Beijing Kwinbon Biotechnology Co., Ltd., Beijing 102206, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.02.008 |
| 年份 | 2026 |
| 刊期 | 2 |
| 页码 | 42-45 |
| 关键词 | Pork; Liquid chromatography-time-of-flight mass spectrometry (LC-TOF/MS); Prohibited veterinary drugs |
| 摘要 | [Objectives] This study was conducted to establish an analytical method for the determination of 14 veterinary drug residues in pork using liquid chromatography-time-of-flight mass spectrometry (LC-TOF/MS). [Methods] The separation was performed on a Phenomenex Kinetex® column. Qualitative analysis was conducted in multiple reaction monitoring (MRM) mode, and quantification was carried out using the matrix-matched external standard method. [Results] Under optimized conditions, the retention times of the 14 prohibited veterinary drugs ranged from 1.2 to 9.0 min. Within the mass concentration range of 5-200 μg/ml, all analytes exhibited good linearity, with correlation coefficients (r) ≥ 0.999 1. The limits of detection (LOD, S/N = 3) ranged from 0.07 to 0.78 μg/kg, and the limits of quantification (LOQ) ranged from 0.25 to 1.98 μg/kg. The average recoveries at three spiked concentration levels ranged from 82.4% to 104.8%, with relative standard deviations (n = 6) between 2.8% and 6.9%. [Conclusions] This method is simple and accurate, and enables rapid qualitative and quantitative analysis of 14 prohibited veterinary drug residues in pork. |
