| 刊名 | Agricultural Biotechnology |
| 作者 | Benwei SU1,2#, Hailin LU3#, Lizhang LI3, Jiali LIU2, Kaixin ZHU2, Zishu CHAI1,4* |
| 作者单位 | 1.Faculty of Chinese Medicine Science of Guangxi University of Chinese Medicine, Nanning 530200, China; 2. Qinzhou Hospital of Traditional Chinese Medicine, Qinzhou 535000, China; 3. Guangxi University of Chinese Medicine, Nanning 530200, China; 4. Guangxi Cenxi Taxillus chinensis Science and Technology Backyard, Wuzhou 54300, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.02.011 |
| 年份 | 2026 |
| 刊期 | 2 |
| 页码 | 61-68 |
| 关键词 | Taxillus chinensis; Quercitrin; 1-Deoxynojirimycin; Qualitative and quantitative detection; Quality control |
| 摘要 | [Objectives] This study was conducted to establish a quality control method for mulberry-parasitizing Taxillus chinensis by detecting both quercitrin, an inherent component of the medicinal material, and 1-deoxynojirimycin (1-DNJ), a characteristic component derived from the mulberry host, using a "dual-substance detection" approach. [Methods] Ten batches of mulberry-parasitizing T. chinensis samples from different producing areas were collected, and T. chinensis samples parasitizing oil-tea camellia and oleander were used as non-mulberry host controls. TLC was used for qualitative identification of quercitrin and 1-DNJ in the samples. HPLC-UV was employed for quantitative determination of quercitrin, and HPLC-ELSD was used for quantitative determination of 1-DNJ. [Results] Under UV light at 365 nm, distinct quercitrin spots (Rf = 0.46) were observed in all ten batches of mulberry-parasitizing Taxillus chinensis samples and in the two control samples (Taxillus chinensis parasitizing oil-tea camellia and oleander). In contrast, no corresponding quercitrin spots were detected in the host samples (mulberry branches, oil-tea camellia branches, and oleander branches). Under natural light, distinct 1-DNJ spots (Rf = 0.324) were observed in all ten batches of mulberry-parasitizing T. chinensis samples and in their host mulberry branches. In contrast, no corresponding 1-DNJ spots were detected in T. chinensis samples parasitizing oil-tea camellia or oleander, nor in their host branches (oil-tea camellia branches and oleander branches). The average recovery of quercitrin was 99.5% (RSD = 2.95%). The average quercitrin content in the ten batches of mulberry-parasitizing T. chinensis samples ranged from 1.98 to 3.11 mg/g, while no quercitrin was detected in the host mulberry branch samples. The average recovery of 1-DNJ was 98.03% (RSD = 1.15%). The 1-DNJ content in the ten batches of mulberry-parasitizing T. chinensis samples ranged from 1.35 to 5.08 mg/g, while that in the host mulberry branches ranged from 3.21 to 9.41 mg/g. No 1-DNJ was detected in T. chinensis samples parasitizing oil-tea camellia or oleander, nor in their host branches (oil-tea camellia branches and oleander branches). [Conclusions] The detection method based on the "dual-component" approach using quercitrin and 1-DNJ is highly specific and simple to operate. It enables both the identification of the host origin and the quality control of mulberry-parasitizing T. chinensis. This "dual-component" quality control method has significant implications for guiding the quality control of parasitic herbs derived from other host plants. |
