| 刊名 | Agricultural Biotechnology |
| 作者 | Zhenghua LIU;Zhonghai |
| 作者单位 | 1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China; 2. Technology Center of Changsha Customs District of People's Republic of China, Changsha, 410004, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.01.013 |
| 年份 | 2024 |
| 刊期 | 1 |
| 页码 | 51-57 |
| 关键词 | G-quadruplex/hemin DNAzyme; Multivalence; Catalytic hairpin assembly; Exonuclease III; Signal amplification; Colorimetric detection |
| 摘要 | Mercury ion (Hg2+), a highly noxious of heavy metalion, has detrimental effects on the ecological environment and human health. Herein, we have developed an exonuclease III (Exo III) assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg2+. A hairpin DNA (Hr) was designed with thymine-Hg2+-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg2+. A released DNA fragment triggers the catalytic assembly of other three hairpins (H1, H2, and H3) to form many trivalent G-quadruplex/hemin DNA enzymes for signal output. The developed sensor shows a dynamic range from 2 pM to 2 μM, with an impressively low detection limit of 0.32 pM for Hg2+ detection. Such a sensor also has good selectivity toward Hg2+ detection in the presence of other common metal ions. This strategy shows the great potential for visual detection with portable type. |
