| 刊名 | Agricultural Biotechnology | 
| 作者 | Yuxuan WU, Junqing WANG, Wenjie WANG | 
| 作者单位 | College of Bioengineering,Qilu University of Technology (Shandong Academy of Sciences),Jinan Baotuquan Liquor-making Co. | 
| DOI | DOI:10.19759/j.cnki.2164-4993.2023.03.024 | 
| 年份 | 2023 | 
| 刊期 | 3 | 
| 页码 | 106-112 | 
| 关键词 | Aqueous two-phase system; Ultrasound assistance; Response surface optimization; Sweet potato leaf polysaccharides | 
| 摘要 | [Objectives]The ultrasound-assisted aqueous two-phase extraction of sweet potato leaf polysaccharides was studied.[Methods]With the yield of sweet potato leaf polysaccharides as the index,the aqueous two-phase extraction system was determined,and the optimal extraction conditions were optimized by single-factor experiments and response surface methodology.[Results]The optimal parameters were ethanol concentration 25.68%,liquid-to-material ratio 55.83,and ultrasonic treatment time 38.33 min.Under these conditions,the yield of sweet potato leaf polysaccharides could reach 20.646 mg/g.[Conclusions]The ethanol/ammonium sulfate aqueous system is a rapid and efficient method for extracting sweet potato leaf polysaccharides,which is of great significance for the application of sweet potato leaf extract as a natural food additive. |