| 刊名 | Agricultural Biotechnology | 
| 作者 | Guofeng YU2, Cuishu LENG2, Keli FANG3, Zhiguang LIU2, Jianhong SUN2,Zengyu Wang2, Xuegang LI1,Shuai Zhang2, Jian LIU1, Xulun WEI1, Qiulin LIU1, Shoujie NIE1* | 
| 作者单位 | 1. Yizhong (Qingdao) Industrial Co., Ltd., Qingdao 266021, China; 2. Technical Center, China Tobacco Shandong Industrial Co., Ltd., Qingdao 266101, China; | 
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.03.007 | 
| 年份 | 2024 | 
| 刊期 | 3 | 
| 页码 | 24-27 | 
| 关键词 | Combined application of ultrasonic wave and 4C technique; Orthogonal experiment; Formula extraction; Flavor | 
| 摘要 | [Objectives] The extraction conditions of formula oolong tea was investigated by an orthogonal experiment. [Methods] The technical conditions were optimized by the 4C method, and the application of formula oolong tea extract in cigarettes was studied. [Results] ① In the experimental range, the best sensory evaluation effect of formula oolong tea extract was obtained with extraction conditions of 70% ethanol as extraction solvent, extraction time h, extraction temperature 25℃, and ultrasonic frequency 80 kHz, and follow-up low-temperature concentration, low-temperature sedimentation and low-temperature centrifugation. ② The effects of different centrifugal speeds on the quality of formula oolong tea extract were explored. The formula oolong tea extract obtained under the conditions of 3 000 r/min and centrifugal time of 10 min showed the best evaluation effect with soft and delicate smoke, rich smoke fragrance, good comfort and refreshing mouthfeel. ③ The effective aroma components in the formula oolong tea extract were qualitatively analyzed by GC-MS. [Conclusions] This study provides high-quality raw materials and a theoretical basis for the research of independent flavor blending in cigarette industry enterprises. |