| 摘要 |
［Objectives］ To establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of forsythin, baicalin, and chlorogenic acid in a traditional Chinese medicine spray disinfectant. [Methods] The chromatographic separation was performed on a GL Sciences (19H0044724)-C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of acetonitrile -0.05% formic acid solution at a flow rate of 1 mL/min. The injection volume was 10 μL, detection wavelength was set at 280 nm, and column temperature was maintained at 25 ℃. [Results] The linear ranges of forsythin, baicalin, and chlorogenic acid were 10.5-52.5, 20.6-103, and 14.2-71 μg/mL, respectively, with good linear relationships between concentration and peak area (R2=0.999 9). The relative standard deviations (RSDs) for precision and repeatability tests were all ≤1.0%. The average recoveries were 98.51%, 98.48%, and 97.71% for the three components, with RSDs of 0.96%, 0.97%, and 0.73%, respectively. [Conclusions] This method demonstrates strong specificity, high precision, excellent accuracy, and simplicity of operation, making it suitable for the simultaneous quantification of forsythin, baicalin, and chlorogenic acid in traditional Chinese medicine spray disinfectants. It provides a reliable basis for quality control and practical applications in animal breeding environments. |
