| 摘要 |
［Objectives］ To optimize the optimal extraction process of Qingdu Jianpi Mixture. [Methods] Taking water addition ratio, extraction time and extraction times as process investigation factors, psoralen content, astilbin content and dry extract yield as evaluation indicators, the main influencing factors and level range of the extraction process of Qingdu Jianpi Mixture were determined on the basis of single factor test method, and the optimal weight coefficient was screened by AHP-entropy method mixed with weighting method. Combined with L9 (34) orthogonal experiment, the best extraction process was obtained. At the same time, thin-layer chromatographic identification was used to identify Ficus simplicissima Lour. and Smilax glabra Roxb. in the medicinal liquid. [Results] The best extraction process: add 1：12 water to the prescription decoction pieces, extract under reflux for 2 times, 1.5 h per time, and combine the filtrate to 250 mL. Thin layer chromatography analysis showed that the spots of Ficus simplicissima Lour. and Smilax glabra Roxb. in the medicinal solution were the same as those of reference substances at the corresponding positions, and the negative control had no interference. [Conclusions] The experimental method is reasonable and feasible, and the process is reliable, which can provide experimental reference for the subsequent application of in-hospital preparations and research and development of Qingdu Jianpi Mixture. |
