| 摘要 |
［Objectives］ To investigate the effects of various extract fractions of Hemsleya sphaerocarpa and their mechanisms of action on hepatotoxicity in normal experimental mice and to provide a scientific foundation for the clinical application of H. sphaerocarpa. [Methods] The extracts were separated by vacuum rotary evaporation into aqueous, n-butanol, petroleum ether, and ethyl acetate fractions. The resulting extracts from various fractions were subsequently formulated into 10% drug solutions using a normal saline solution. These solutions were administered intragastrically to mice at a dosage of 0.1 mL/10 g once daily. After 14 d of intragastric administration, various indices were assessed, and serum samples were collected from the ocular region of the mice for analysis. [Results] 15 g of the aqueous fraction, 20 g of the n-butanol fraction, 30 g of the petroleum ether fraction, and 20 g of the ethyl acetate fraction were obtained through the vacuum rotary evaporation method. After 14 d of intragastric administration, the serum biochemical indices of the mice were assessed. It was observed that alanine aminotransferase (ALT) levels significantly increased in the mice of experimental group. This finding suggests that the ethyl acetate, petroleum ether, and n-butanol extracts of H. sphaerocarpa may contribute to liver injury in the subjects. After 14 d of drug withdrawal, the ALT level in the ethyl acetate group exhibited a significant decrease, but remained elevated compared to those in the normal saline group. In contrast, the ALT levels in the n-butanol and petroleum ether groups also demonstrated a significant reduction and were marginally lower than those observed in the normal saline group. Furthermore, the body weights of the mice in both the petroleum ether and n-butanol groups did not show any significant changes throughout the duration of drug administration. [Conclusions] The liver injury in mice induced by the ethyl acetate extract of H. sphaerocarpa is characterized as the most severe and challenging to self-heal. |
