联系方式
电话: 0551-65148112
E-mail: asiaar@163.com
asiaar2009@vip.163.com
QQ: 434421224
104693920
2438070182
友情链接
最新文章
Coupling and Coordination of Digital Economy and High-quality Agricultural Development: A Case Study of Shaanxi Province
摘要: This study, taking Shaanxi Province as an example, systematically analyzed the coupling and coordination relationship between the digital economy and high-quality agricultural development from 2015 to 2024 by comprehensively employing the coupling coordination model and the entropy method. The findings reveal that both the digital economy and high-quality agricultural development indices in Shaanxi Province exhibit a consistent upward trend. The level of coupling coordination has progressively transitioned from a low degree of coupling to a coordinated development stage, establishing a virtuous interactive pattern characterized by "digital enabling agriculture" and "agriculture driving innovation". The driving force behind this coupling primarily stems from the synergistic interplay of a triple mechanism involving "policy guidance, technological breakthroughs, and industrial support". However, structural imbalances persist across regions and agricultural segments, chiefly manifested as the digital divide between the Guanzhong Plain and the Northern (Shaanbei) and Southern (Shaannan) regions, alongside insufficient integration depth of digital technologies in the latter stages of the agricultural industry chain. To address these challenges, the study proposes recommendations including building a "tri-chain integrated" technological ecosystem, optimizing a differentiated policy support mechanism, and implementing regionally targeted strategies. These measures aim to foster deep integration of the digital economy with the entire agricultural value chain across diverse regions, offering pathways for western agricultural provinces to bridge the digital divide and achieve rural revitalization.
关键词: Digital economy, High-quality agricultural development, Coupling coordination
Effects of Cultivation Methods on Yield and Quality of Radix scutellariae
摘要: [Objectives] To investigate the effects of three cultivation methods-level bed, high ridge, and ridge cultivation-on the yield and quality of Radix scutellariae, providing a basis for establishing its Good Agricultural Practice (GAP) technical system. [Methods] Utilizing two-year-old R. scutellariae plants from the cultivation base in Kushan Township, Ju County as the experimental material, yield-related indicators including root length, root diameter, branch number, and fresh weight were measured at harvest time. The baicalin content in the roots was determined using High-Performance Liquid Chromatography (HPLC), and the differences in effects among the cultivation methods were analyzed. [Results] R. scutellariae cultivated using the ridge method exhibited significantly superior root length compared to both level bed and high ridge cultivation. Its branch number was significantly higher than that under level bed cultivation. The baicalin content reached 13.17%, surpassing that achieved with high ridge (12.73%) and level bed (11.87%) cultivation. Based on a comprehensive evaluation of agronomic traits and active constituent content, R. scutellariae cultivated using the ridge method demonstrated significant superiority in both yield and quality over those grown under high ridge or level bed conditions. [Conclusions] Ridge cultivation effectively promotes root growth in R. scutellariae, enhances medicinal material yield and baicalin content. This method is recommended for widespread application in artificial cultivation and provides a scientific foundation for establishing R. scutellariae GAP standards.
关键词: Radix scutellariae, Baicalin, Cultivation method, HPLC
Cloning and Bioinformatic Analysis of the fbpA Gene in Vibrio alginolyticus
摘要: [Objectives] To clone the fbpA gene from Vibrio alginolyticus strain HY9901 and perform bioinformatic analyses, aiming to preliminarily elucidate the structure and function of the FbpA protein. [Methods] The fbpA gene was amplified using PCR and sequenced. Bioinformatics software was employed to analyze the fbpA gene sequence and the deduced FbpA protein for physicochemical properties, signal peptides, transmembrane structures, functional sites, subcellular localization, homology comparisons, phylogenetic analysis, and structural prediction. [Results] The fbpA gene (1 014 bp, encoding 337 amino acids, GenBank accession number PP707017) was successfully cloned. The FbpA protein was identified as a stable hydrophilic protein (molecular weight 37.589 kD, pI 5.97) containing a signal peptide, lacking transmembrane domains, and predicted to be localized extracellularly. It harbors 7 N-myristoylation sites, 8 phosphorylation sites, 2 N-glycosylation sites, and 7 microbody C-terminal targeting signal sites. The protein is highly conserved within the Vibrio genus, exhibiting 99.41% and 99.40% similarity to the Vibrio diabolicus subgroup and Vibrio antiquarius, respectively, with all three clustering together on the same evolutionary branch. Secondary structure prediction indicated a predominance of alpha-helices (49.85%) and random coils (30.27%). [Conclusions] This study successfully cloned the fbpA gene and characterized the structural features and evolutionary relationships of the FbpA protein, laying a foundation for further investigation into its role in the pathogenesis of V. alginolyticus and the development of vaccines.
关键词: Vibrio alginolyticus, Gene amplification, fbpA gene, Bioinformatics analysis
Expression Analysis and Transcription Factor Prediction of Fibroblast Growth Factor 10 During Differentiation of Rat L6 Myoblasts
摘要: [Objectives] To characterize the expression pattern of Fibroblast growth factor 10 (FGF10) during the differentiation of rat L6 myoblasts and to identify potential key transcription factors (TFs) regulating its expression through bioinformatics approaches. [Methods] Rat L6 myoblasts were induced to differentiate by culturing them in DMEM supplemented with 2% donor horse serum (DHS). Morphological changes were observed using an inverted microscope. Cell samples were collected prior to induction (day 0) and on days 1, 3, 5, and 7 post-induction. The relative expression levels of FGF10 mRNA and protein at each time point were quantified using RT-qPCR and Western blot analysis, respectively. Furthermore, a 2 000 bp sequence upstream of the transcription start site of the rat Fgf10 gene was extracted as the promoter region. Putative TF binding sites were predicted using four databases (TRANSFAC, JASPAR, HOCOMOCO, and CISBP), and high-confidence candidates were screened to construct a regulatory network. [Results] Morphological observations confirmed successful differentiation, as evidenced by the appearance of binucleated myotubes on day 3 and the formation of numerous thick, multinucleated myotubes by day 7. Both RT-qPCR and Western blot analysis demonstrated a significant dynamic expression pattern of FGF10. Expression levels were markedly upregulated during the early phase (days 1-3), reaching a peak on day 3 (P<0.01), followed by a decline to basal levels during the late phase (days 5-7). Cross-validation across multiple databases identified 48 high-confidence TFs, among which Elf5, Tcf3, Nkx3-2, Zic2, Tcf7, and Egr1 were consistently predicted by all four databases. [Conclusions] FGF10 exhibits high expression levels during the early stage of differentiation, indicating its crucial role in the initiation of myogenesis. The six identified TFs serve as core candidate regulators of Fgf10 expression, offering novel insights into the molecular mechanisms underlying muscle development.
关键词: L6 myoblast, Differentiation, FGF10, Transcription factor, Expression pattern